Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. In addition, the SpectraMax iD5The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Filters: Excitation and emission slides for 4 filters each. as a fluorescence blank. FAST AND ACCURATE METERTECH Microplate Reader $3,650. View online or download Molecular devices SpectraMax M3 User Manual. Type. It allows you to explore cellular pathways and protein activation and expression in one system. 1. Fluorescence Microplate Reader Comparison Chart. S. With optimized reagents, option to operate the. 1. SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190. The SpectraMax iD5 reader rapidly performs absorbance measurements that are used to plot standard curves and calculate sample concentrations using a preconfigured protocol in SoftMax Pro Software. The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. BMG Labtech - THERMOstar. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The plate reader optics are tunable for absorbance. The cooled PMT detector allows high sensitivity in a wide dynamic range, even in extremely low light. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Fluorescence is measured at time zero and again after one-hour of incubation at 37°C in a fluorescence microplate reader. To avoid these issues and provide the best possible dynamic range between the highest and the lowest measurement values of your assay, fluorescence gain is typically adjusted on the sample with the expected highest signal output (e. Plate chamber design of the SpectraMax category 1 microplate readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Calcein AM is a widely used live-cell marker. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. In addition, the SpectraMax iD5Popular answers (1) If you are going to read the plate from the top, you should use a solid black plate. Luminescence, and Fluorescence microplate re aders—providing extensive data calculation. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a. Plate reader. E Models. 验证检测 SpectraMax 190 的工作状态。 堆板机、机械臂一体化整合 SpectraMax 190 可以在几分钟内整合到 Molecular Devices 的 StakMax 堆板机上。为了实现更高程度的自动化，自动化供应商 合作伙伴已经把我们的酶标仪系统和所有主要合作伙伴的机械臂 进行了整合。I am interested in using a SpectraMax M2 Plate Reader for a spectrophotometric assay and am trying to collect the proper reagents and materials. Item Description Sensitivity 10amol/wellalkalinephosphate200µL Wavelengthrange 250-850nm Crosstalk <0. Select the Driver tab and click Update Driver. In the M2, M5 and Plus 384, the light. The protocol contains settings for reading the plate, as well as template groups that allow a user to easily set up analysis, including calculation. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. When a feature or capability applies to only certain readers, this exception is noted. Because. A microplate reader as individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Listing: #4772697. 00. This opens the Settings window. Factors that contribute to an instrument’s sensitivity, or limit of detection, include background noise from the detector and electronics, the type of detector that is used, and the instrument’s configuration and. (See Figure 7. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. luminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. Maximum signal with lowest background. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. 00. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. Skip to main content. Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. Microplate Capacity. WinKQCL ® Software can be used with a compatible microplate reader to run the template and. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. $ 9,499. 1. SpectraMax ® M2/M2e: Not Capable: Validated:. The percent The percent (4) Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant behaviour by TRAF6/NF-κB inhibition Pharmaceutical Biology December 31, 2022 Dang-ke. Download Application Note Plate reader settings for NanoOrange assay. Using a black. I understand the OD measurement in a plate reader varies with the volume of the sample measured. 25 to 3. Butyrate (5 μM, 24h), doxorubicin (1 μM, 2h) and H 2 O 2 (2 mM. Fill a clean plate with water. All SpectraMax® M series multi-mode microplate readers measure UV and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide. (a) Chemical structures of 1 (HADP) and its analogues 2–4. The SpectraMax® M2 and M2e Multimode. Fluorescent Polarization: Solid black plates, as above. A decrease in fluorescence with increasing staurosporine concentration was detected with the SpectraMax iD3 reader. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2. Traditional microplate readers perform mostly biochemical assays—measuring how much light passes through or is emitted by a sample. 9000-0756. BioTek’s Cytation™3, for instance, is a multimode plate reader that also functions as a high-speed automated digital fluorescence microscope with brightfield capability. The assay is carried out in a standard 96-well plate. The SPECTRAmax® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics that permit experimental determinations at low-level detection limits. Plate Readers. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Tecan’s. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. A whole well fluorescence imaging system that can collect information from all cells inside the well, from 6- to 384-well formats. patent nos. Enables accurate fluorescence data with every run. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Peeking into a research lab today, it would be rare to see a line-up of microplate readers on a bench, each capable of performing only a single type of measurement. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Absorbance cuvette port. SpectraMax® M2 SpectraMax® M2e Multi-Mode Microplate Readers User Guide 0112-0102 E October 2018 SpectraMax M2 and M2e Microplate Reader User. • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. Measure more accurately. Microplate Readers. Qualify the fluorescence performance of the SpectraMax . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 0 out of 5 $ 7,699. 7°C (gradient heating design). SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Figure 2. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. Microplate readers’ standard detection modes are. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. Analyze the dataHere we report the use of the EarlyTox ™ Live/Dead Assay Kit in combination with SpectraMax ® fluorescence microplate readers (Figure 1). Fluorescence measurements were acquired at 10 min intervals (ex: 485 nm and em: 535 nm) in a SpectraMax M2 fluorescence plate reader (Molecular Devices) operated in the 384-well microplate format. 0 software was used to handle data analysis and control each instrument. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. spr SpectraMax iD5 SpectraTest ABS2 v1. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Plate reader set to 50 reads. In addition, the SpectraMax iD5SpectraMax Microplate Readers. Fluorescence measurements no longer require you to adjust the gain. 5008531B 3 Contents Chapter1:SpectraTestMulti-ModeValidationPlateOverview 4 Multi-ModeProtocols 6 Chapter2:GettingStarted 8 CertificateofCalibration 8SpectraMax M2. For example, microplates containing different samp les can be read using the same orThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The chamber contains. SpectraMax ® M2 Multi-Mode Microplate Reader. Deviations in boronic acid concentration were necessary when the K ARS for a boronic acid was too low (<100 M. The spectrophotometric performance of the SpectraMax M2 and SpectraMax M2e is similar to the SpectraMax Plus, a dedicated absorbance plate reader. 020 OD 2. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. STAKMAX. An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. 40266 Email: drugdiscoverytech@lifetech. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Reading Capabilities: cuvette or microplate. Absorbance (monochromator) Fluorescence (monochromator; FI). The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. The plate reader. on SpectraMax M2 fluorescence plate reader (Molecular Devices, CA, USA): excitation wavelength 320nm, emission wavelength 470nm. BMG Labtech - THERMOstar. 25 fmol/well 384-well plate) Filters/Mirrors: Fluorescein 1 pM typical (0. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader Absorbance ü ü ü ü Fluorescence Intensity ü ü ü ü Luminescence ü ü ü ü Time-Resolved Fluorescence ü ü ü Fluorescence Polarization ü ü HTRF ü Upgrade Options TRF, HTRF, FP HTRF, FP HTRF N/A Plate Formats 6-, 12-, 24-, 48-, 96-, 384. Perkin Elmer Envision. FI assay. MOLECULAR DEVICES SPECTRA MAX GEMINI XS MICRO. ) Identical data and curves were generated from Gemini EM and SpectraMax M2 but are not shown here. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch. Molecular Devices SpectraMax M2 Microplate Reader. Temperature variation ± 0. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. The FI assay was performed using the SpectraMax ® M2 Multi-Mode Microplate Reader. Fluorescence Microplate Reader Comparison Chart. I've got a LT4500 microplate reader and was hoping to digest the pellets in papain overnight, simply mix a measure of that sample with DMMB solution in a 96 well plate and read absorbance at 525nm. Documentation:Plate readers that are used to detect luminescence, fluorescence and absorbance signals can vary in sensitivity (Figure 2). SpectraMax M2 and M2e Multi-Mode Microplate Readers User Guide Molecular Devices SpectraMax M2 and M2e Multi-Mode Microplate Readers. WinKQCL ® Software guides the user through the process of setting up a microplate template and running an assay to determine the amount of endotoxin in a sample solution. 7°C and the bottom to 36. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 reader measures absorbance, fluorescence, and luminescence. Adjusting fluorescence gain on microplate readers. 53 - 60 kg, (117-132 lb), depending on configuration. 2. This enables the standard curve to be plotted and sample concentrations to be calculated automatically by SoftMax. Get unrivalled sensitivity and reproducibility and elevate your microplate assay protocol with the Eppendorf Assay/Reader Plates. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. The SpectraMax i3 Multi-Mode Microplate Reader and the SpectraMax MiniMax 300 Imaging Cytometer enable both ways of assessing transfection conditions for GFPbased reporter assays. The software is easy to use and contains settings for hundreds of commonly used assays, such as BCA and MTT. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Step 4: Use the Template Editor to assign wells to ‘Standards’ and ‘Unknowns’ groups (Figure 2). The following general steps are taken with our absorbance and fluorescence readers. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Plate stacker and robot integration The SpectraMax M2 and M2e readers can be integrated with the A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Prior to fluorescence analysis, the lid from the OmniTray plate was removed. Dose-dependent enzyme essay Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Molecular devices SpectraMax M3 Pdf User Manuals. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. The purple holder is used for reading non-transparent plates from the top (e. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Dual monochromators allow selection of any absorbance wavelength between 200 nm and 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Instruments are configurable for the assay-specific detection modes and performance your laboratory needs. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF. Cells in each image are identified by SoftMax Pro Software and cell-by-cellfluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). 67. Conclusions. Choose from a three- (M3), four- (M4), orThere were 12 replicate wells per dilution in both 96-well and 384- well plates. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and. S. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The kit includes everything you need: reagents, buffers and a microplate. (b) Fluorescence responses of probe HADP and control compounds. Microplate Formats: 6, 12, 24, 48, 96, 384. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. (12. Category 2 and 3 microplate readers use gradient heating as well. Three data types for FP read types are also being introduced with v4. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. White plates reflect the light, enabling even low levels of Luminescence to be detected. Note: Check the Applies to section at the bottom of the page to make this is the proper driver for your plate reader. Parameter EarlyTox Live/Dead EarlyTox Glutathione EarlyTox Caspase-3/7 R110 Read mode Fluorescence Fluorescence Fluorescence Read type Endpoint Endpoint Endpoint Wavelengths Lm1:. 2. 0 out of 5 $ 3,249. Figure 2-23: Bottle holder To install the bottle holder: 1. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators. Third this recommendation. The SpectraMax® ABS Plus Microplate Reader can run . When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. In simplest terms, a plate reader measures and records a signal produced from the wells of a multiwell plate. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. If a lab requires instruments to read ELISA plates, one to do fluorescence and one for luminescence, it will likely look for one instrument that can handle all these. The SpectraMax Paradigm Detection Cartridge design utilizes an energy intensity optimization technique that allows the user to read up to seven logs of sample concentration in a single pass of a plate without any manual intervention. Auto-calibration and self-diagnostics. Avoid losing high signal. 0 × 107) RLU 0. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. It is typically used to determine the concentration of nucleic acids and proteins. Plate reader instructions Computer. 1 fmol/well 384-well plate) Luminescence Specifications Wavelength Range 300 to 700 nm Dynamic Range < 6 decades Sensitivity 10 amol ATP typical (flash) Fluorescence Polarization Specifications Light Source. 0) OD (500 to 1100) RFU (5. Add to Cart. 0 × 103 to 2. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 2. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. 627. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. It is particularly powerful when monitoring fast kinetic assays in a high. Dose-dependent enzyme essayVisualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Add to Cart. spr SpectraMax iD3 SpectraTest ABS2 v1. dual monochRomatoRs foR assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. Align the two slots on the bottle holder with the two pegs on the rail. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. The fluorescence was measured in the SpectraMax M3 Microplate reader. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader Absorbance ü ü ü ü Fluorescence Intensity ü ü ü ü Luminescence ü ü ü ü Time-Resolved Fluorescence ü ü ü Fluorescence Polarization ü ü HTRF ü Upgrade Options TRF, HTRF, FP HTRF, FP HTRF N/A Plate Formats 6-, 12-, 24-, 48-, 96-, 384. For example, microplates containing different samples can be read using the Tecan Infinite® M1000 Pro Plate Reader. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Fluorescence Microplate Reader Comparison Chart. com 10. PC software (SkanIt software) Dimensions (D x W x H) 58 x 53 x 51 cm (23 x 21 x 20 in) Weight. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. However, proNGF-induced receptor complex formation has been difficult to directly assess other than by western blotting. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. variety of. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 5%in96-and384-wellmicroplates. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Category 2 and 3 microplate readers use gradient heating as well. Plate Shaking Linear, orbital, double-orbital Dimensions (H x W x D) 12 x 15 x 15 in. The chamber. The SpectraMax iD3 reader measures absorbance, fluorescence, and luminescence. Figure 6: Transcreener ADP2 FI assay principle. By employing dyes that specifically bind to nucleic acids, DNA/RNA concentrations can also be quantified using fluorescence detection. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 6 % of reading 4. standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax M2 and use thereof is covered by issued (U. ZERO BIAS - scores, article reviews, protocol conditions and moreFluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. The instrument will read the plate, then the relative fluorescence units (RFUs) will be displayed in the Plate section of SoftMax Pro Software. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. 0) OD (500 to 1100) RFU (5. Reader Documentation Version No. Luminex Magpix. Spectramax Multi-Mode Microplate Reader Performance Specifications. 2. Table 1. The Spectramax i3 is a muti-mode plate reader used for measuring absorbance and fluorescence in microtiter plates. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. MPN: 50505. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. Shop all used microplate readers and parts from a variety of manufacturers. Select the lab user profile. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. In the presence of ACE, the PL intensity was increasing by time, while the PL intensity sustained a low and stable fluorescence in 3 hours in the absence of ACE. In the presence of ACE, the PL intensity was increasing by time, while the PL intensity sustained a low and stable fluorescence in 3 hours in the absence of ACE. a multi-detection microplate reader with two-mode cuvette port Spectramax® m2/m2e user guide SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM SpectraMax M2 & SpectraMax…plate and push the “drawer” button (this does not need to be done before opening/exploring the software, but must be done before reading the plate, of course). When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. ID: MOLECULAR DEVICES SpectraMax M2 is a multi-mode microplate spectrophotometer providing unbeatable accuracy, dependability and versatility for any lab experiment, with a range of detection modes, advanced accessories, and intuitive software. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (plate reader can measure multiple readouts, including UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 0077 OD 0. . fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). SpectraMax fluorescence microplate reader with SoftMax® Pro Software, is a quick, sensitive detection method for proteins. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Data from The SpectraMax® iD3 Multi Mode Microplate . $ 2,499. SpectraMax M2 and use thereof is covered by issued (U. Transcreener ® ADP. for the plate. These robust readers have been placed in labs from Antarctica to the. Add to Cart. Add to Cart. We suggest Corning 384-well plates Cat. 8 is required to operate the new SpectraMax® M5 reader and its five read types: Fluorescence Intensity, Absorbance, Luminescence, Time-Resolved Fluorescence (TRF) and Fluorescence Polarization. The password is. okyo M LLC O T rinted in in rinted P. Synergy H1 is a modular multimode microplate reader, with monochromator-based and filteru0002based optics. In the lower half of the window, left side, there is a box labeled reader. The SpectraMax iD3 reader detects fluorescence using monochromators only. SpectraMax M5), place the adapter in the plate carriage. free tracer decreases, resulting in an overall increase in RFU values. (a) Chemical structures of 1 (HADP) and its analogues 2–4. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. 0) OD (0. 303 unable to cal dark current. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader andMicroplate Readers. 1 New Reader Support 1. This is a result of the light being attenuated and scattered by the plastics of the well-bottom which can. Perform the scan and note the wavelength that gives maximum emission. It does not work as expected. When reading optical density at wavelengths below 340 nm, special UVtransparent,. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. D 3 1. 6 % of reading 4. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. After the plate is read, data will appear in the plate. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single Fig 2: Enzymatic response of synthetic compounds. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Also refer to this Multimode Reader Comparison Chart. You should use solid white plates for top Time- Resolved Fluorescence reads, and white plates with clear bottoms for bottom reads. 25 to 3. Add to Cart. Reviews. 5,112,134 5,766,875. the reader’s stated range. With-dye, Continual Mode Assay on a SpectraMax M2 Plate Reader. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. This can increase throughput and efficiency, thus saving time and space in the lab. Add to Cart. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Appendix. Microplate Readers Molecular Devices Spectramax M3 Multi Mode Microplate Reader. The instrument is located in room 2131 at 300 George Street. 5 pM typical (0. CA$14,500. With exceptional signal-to-noise ratio and a lower detection limit. Step 5: Click the software’s Read button. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. These lab workhorses are used in drug discovery.